Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase
Experimental & Molecular Medicine
;
: 187-192, 2000.
Artículo
en Inglés
| WPRIM
| ID: wpr-25125
ABSTRACT
Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal alpha-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Células HeLa
/
Exones
/
Empalme Alternativo
/
ADN Complementario
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Lisosomas
/
Manosidasas
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2000
Tipo del documento:
Artículo
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