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Role of progesterone in TLR4-MyD88-dependent signaling pathway in pre-eclampsia / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 730-734, 2013.
Artículo en Inglés | WPRIM | ID: wpr-251402
ABSTRACT
The role of progesterone in the Toll-like receptor 4 (TLR4)-MyD88-dependent signaling pathway in pre-eclampsia was studied. Peripheral blood mononuclear cells (PBMCs) from pre-eclampsia (PE) patients were subjected to primary culture, and stimulated with different concentrations of progesterone (0, 10(-8), 10(-6), and 10(-4) mol/L). The mRNA expression of TLR4, MyD88 and nuclear factor-kappaB (NF-κB) was detected by using real-time PCR. The Ikappa-B protein expression was detected by using Western blotting. The expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the supernatant was determined by using ELISA. With the concentrations of progesterone increasing, the mRNA expression levels of TLR4, MyD88 and NF-κB in 2(-&Delta;&Delta;CT) value were significantly decreased, and the IkappaB protein expression levels were significantly increased. The TNF-α and IL-6 expression showed a downward trend when the progesterone concentration increased, and there were significant differences among all of the groups (P<0.05). It was suggested that progesterone can inhibit the TLR4-MyD88-dependent signaling pathway in PE significantly and benefit for the pregnancy.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Preeclampsia / Progesterona / Sangre / Ensayo de Inmunoadsorción Enzimática / Leucocitos Mononucleares / Transducción de Señal / Expresión Génica / Células Cultivadas / Western Blotting Límite: Adulto / Femenino / Humanos / Embarazo Idioma: Inglés Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Preeclampsia / Progesterona / Sangre / Ensayo de Inmunoadsorción Enzimática / Leucocitos Mononucleares / Transducción de Señal / Expresión Génica / Células Cultivadas / Western Blotting Límite: Adulto / Femenino / Humanos / Embarazo Idioma: Inglés Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Año: 2013 Tipo del documento: Artículo