Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 825-829, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-251849
ABSTRACT
<p><b>OBJECTIVE</b>Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins.</p><p><b>METHODS</b>Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was performed with normal saline (group B), vitamin C (group C) and vitamin E (group D). Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline (sample B), vitamin C (sample C) and vitamin E (sample D). Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression.</p><p><b>RESULTS</b>In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes.</p><p><b>CONCLUSION</b>Vitamin C can protect vascular endothelial cells from mannitol-induced injury.</p>
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxidación-Reducción
/
Patología
/
Farmacología
/
Vitaminas
/
Sondas de Oligonucleótidos
/
Química
/
Regulación de la Expresión Génica
/
Apoptosis
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Biología Celular
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Journal of Zhejiang University. Science. B
Año:
2006
Tipo del documento:
Artículo
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