Reversion of multidrug resistance by CIK in K562/ADR cells and its mechanism exploration / 中华血液学杂志
Chinese Journal of Hematology
; (12): 52-56, 2011.
Article
en Zh
| WPRIM
| ID: wpr-252014
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and mechanism of cytokine-induced killer (CIK) cells in reversing multidrug resistance (MDR) and increasing intracellular concentration of adriamycin (ADR) in the K562/ADR cells.</p><p><b>METHODS</b>Peripheral mononuclear cells (MNCs) were isolated from healthy donors and cultured with combined cytokines to generate CIK. The changes of cell phenotype and cytokines secretion of CIK were determined. K562/ADR cells were divided into three groups: ADR in combination CIK (group I), CIK alone (group II) and ADR alone (group III). The viability and proliferation of K562/ADR cells were assayed by MTT assay, the intracellular concentration of ADR and the expression of P-glycoproteins (P-gp) in K562/ADR cells by FCM.</p><p><b>RESULTS</b>The cytotoxicity of ADR in group I was higher than that in group II (P < 0.05). The cytotoxicity was increased with the E/T ratio increasing (P < 0.05) but had no relation with the concentration of ADR in group I (P > 0.05). The expression of P-gp was declined in group I and group II (P > 0.05). The intracellular concentration of ADR in group I was higher than that in group II (P < 0.05), and had no relation with the ADR concentration (P > 0.05).</p><p><b>CONCLUSION</b>Pre-treatment with CIK can increase the cytotoxicity and the intracellular concentration of ADR and decrease the expression of P-gp in K562/ADR cells in the ADR and CIK combination group. Acute leukemia patients would be most likely to benefit from the combination of chemotherapy and CIK therapy.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
/
Resistencia a Múltiples Medicamentos
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Resistencia a Antineoplásicos
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Células K562
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Alergia e Inmunología
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Células Asesinas Inducidas por Citocinas
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Antineoplásicos
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2011
Tipo del documento:
Article