Establishment of three screening models of angiotensin converting enzyme inhibitors / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1366-1369, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-252906
ABSTRACT
<p><b>OBJECTIVE</b>To establish and compare three in vitro screening models of angiotensin converting enzyme inhibitors (ACEI), and provide methodological basis for screening ACEI drugs from Chinese herbal medicine.</p><p><b>METHOD</b>Three screening models were established using rat serum, pure angiotensin converting enzyme (ACE) and crude extract enzyme from rabbit lung as enzyme sources, respectively, with corresponding testing methods, and captopril as the positive drug.</p><p><b>RESULT</b>The IC50 of captopril was 2.30 nmol x L(-1) using rat serum as the enzyme; and 1.04 nmol x L(-1) for ACE pure enzyme; and 1.40 nmol x L(-1) for crude extract enzyme from rabbit lung.</p><p><b>CONCLUSION</b>Results from the three screening models were all in accordance with literature reports. These models can be applied to in vitro pharmaceutical screening. The selection of suitable screening model depend on the experimental situation and the inherent characters of models.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Sangre
/
Inhibidores de la Enzima Convertidora de Angiotensina
/
Medicamentos Herbarios Chinos
/
Ratas Sprague-Dawley
/
Peptidil-Dipeptidasa A
/
Modelos Animales
/
Evaluación Preclínica de Medicamentos
/
Métodos
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Artículo
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