HPLC-TOF-MS analysis of metabolites of Diffusa effective extracts in rats / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze p-coumaril acid, trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.</p><p><b>METHOD</b>Rat blood samples were collected 1.0 h after oral administration of 0.4 g x kg(-1) exocarpium Diffusa effective extracts, and then were analyzed by using HPLC-Q-TOF-MS.</p><p><b>RESULT</b>In rat plasma, only one metabolite was detected. The structure was identified by reference substance to be p-coumaril acid.</p><p><b>CONCLUSION</b>The methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid. This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.</p>