Protective effects and mechanism of Inonotus obliquus on asthmatic mice / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1067-1070, 2011.
Article
en Zh
| WPRIM
| ID: wpr-252940
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective effects and mechanism of ethanol extract of Inonotus obliquus (EEIO) injection on asthmatic mice.</p><p><b>METHOD</b>OVA was injected intraperitoneally and inhaled to produce the asthmatic model. Thirty two mice were randomly divided into four groups: control group, asthma group and I. obliquus groups of high and low dose. The concentrations of IL-4, IL-5, IL-13 and IFN-gamma in BALF, the phosphor-p38 MAPK in lung tissues were respectively measured by ELISA and Western blotting. The number of inflammatory cells in BALF and histopathology changes were observed.</p><p><b>RESULT</b>In asthmatic group, the number of inflammatory cells and the concentrations of IL-4, IL-5, IL-13 in BALF and phospho-p38 MAPK in lung tissue were higher, while IFN-gamma were lower than those in normal control mice (P < 0.05). In I. obliquus group, the number of inflammatory cells, the concentrations of IL-4, IL-5, IL-13 in BALF and phosphor-p38 MAPK in lung tissue were lower, but were higher than those in normal control mice (P < 0.05), and histropathology damage was alleviated significantly. There was no significant difference observed among the efficacies in the I. obliquus groups of high and low dose.</p><p><b>CONCLUSION</b>p38 MAPK may play a role in pathological process of asthma. I. obliquus effectively treats asthma by inhibiting the expression of phosphor-p38 MAPK, correcting the unbalance of IFN-gamma/IL-4 and decreasing the number of inflammatory cells.</p>
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Índice:
WPRIM
Asunto principal:
Patología
/
Farmacología
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Asma
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Basidiomycota
/
Basófilos
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Líquido del Lavado Bronquioalveolar
/
Extractos Vegetales
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Linfocitos
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Química
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Interleucina-4
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Article