The inhibitory effect of chronic lead exposure on CA1 LTP and ERK2 activity in rat hippocampal area / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 326-328, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-253153
ABSTRACT
<p><b>AIM</b>To investigate the effect of chronic lead exposure on rat hippocampal CA1 LTP and alpha-Ca2+ /calmodulin-dependent protein kinase II (alpha-CaM K II) activity in vivo.</p><p><b>METHODS</b>A stimulus bipolar electrode was placed on the Schaffer/Commissural fibers, with extra cellular microelectrode technique to record the population spike (PS) in the CA1 pyramidal, and we observed the changes of PS amplitude before and after the high frequency stimulation (HFS) of lower, mid and higher level lead exposure groups and the control group, respectively. The hippocampal CA1 alpha-CaM K II activity was determined by Western blots by using phosphorylation antibody.</p><p><b>RESULTS</b>The average changes of PS after HFS of the control group, the lower, mid and higher level lead exposure groups were 162.5%, 105.2%, 86.8%, 83.0%, respectively (P < 0.01 vs. control). Defined control a-CaM K II activity as 100, that the lower, mid and higher level lead exposure groups were 62.0 +/- 3.7, 50.8 +/- 4.0, 43.3 +/- 4.1 (P < 0.01).</p><p><b>CONCLUSION</b>Chronic lead exposure can inhibit CA1 LTP in vivo, and the decrease of alpha-CaMK II activity may play an important role in this mechanism.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ratas Wistar
/
Potenciación a Largo Plazo
/
Pruebas de Toxicidad Crónica
/
Toxicidad
/
Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina
/
Región CA1 Hipocampal
/
Plomo
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2006
Tipo del documento:
Artículo
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