Apoptosis of K562 cells induced by extract of Agkistrodon Halys' venom / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 516-519, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-253285
ABSTRACT
The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase (ERK) in K562 cells was detected by Western blot. The results showed that when K562 cells were treated with 0, 1, 10, 20 microg/ml of the extraction for 48 hours, the apoptosis rates were 2.1%, 21.3%, 49.7%, 70.1%, respectively. The proliferation of K562 cells was obviously inhibited in dose-dependent manner. Typical morphologic changes significantly appeared in the extract-treated K562 cells. The extract obviously inhibited the activity of ERK in K562 cells. It is concluded that the extract of Agkistrodon Halys' venom can inhibit the proliferation of K562 cells and induce apoptosis of K562 cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Química
/
Apoptosis
/
Agkistrodon
/
Células K562
/
Venenos de Crotálidos
/
Mezclas Complejas
/
Quinasas MAP Reguladas por Señal Extracelular
/
Proliferación Celular
/
Metabolismo
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS