Foxp3-transfected CD4+ CD25- T cells suppress function of dendritic cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 164-169, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-253359
ABSTRACT
To explore the relationship between expression of Foxp3 gene and immune activity of CD4(+) T cells, the Foxp3 gene was transfected with retroviral vector and applied to forcedly express Foxp3 protein in naive CD4(+)CD25(-) T cells, and then the effect of transfected CD4(+)CD25(-) T cells on immune co-stimulatory molecules and immune function of dendritic cells (DCs) was investigated, and the dependence of direct contact between Foxp3-transfected CD4(+)CD25(-) T cells and DCs was clarified by Transwell test. The results showed that through transfection of retroviral vector, CD4(+)CD25(-) T cells model expressing Foxp3 was established. At 1 week after transfection, proportion of T cells expressing Foxp3 was 38%. CD4(+)CD25(-) T cells forcedly expressing Foxp3 could play immune suppression role in vitro and induce down-regulation of CD80 and CD86 expression on the membrane of DCs. The lymphocyte proliferation test in vitro indicated that Foxp3 transfected CD4(+)CD25(-) T cells could inhibit effect of DCs on activation of allo-lymphocytes. It is concluded that the effect of Foxp3-transfected CD4(+)CD25(-) T cells on DC depends on intercellular direct contact.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Retroviridae
/
Células Dendríticas
/
Transfección
/
Linfocitos T CD4-Positivos
/
Antígenos CD4
/
Linfocitos T Reguladores
/
Alergia e Inmunología
/
Factores de Transcripción Forkhead
/
Subunidad alfa del Receptor de Interleucina-2
/
Vectores Genéticos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2008
Tipo del documento:
Artículo
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