Expression of homeobox gene Msx-1, Msx-2 and Dlx-2 during murine mandibular first molar development / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 429-431, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-253681
ABSTRACT
<p><b>OBJECTIVE</b>To observe the expression of homeobox gene Msx-1, Msx-2 and Dlx-2 during murine mandibular first molar development.</p><p><b>METHODS</b>The murine heads or mandibles on embryonic days 11-18 (E11-18) and postnatal day 1-3 (P1-3) were removed, fixed and embedded, 5 micro m serial sections were cut in the coronal plane. Msx-1, Msx-2 and Dlx-2 RNA probes were synthesized by in vitro transcription and labeled with digoxigenin. Msx-1, Msx-2 and Dlx-2 mRNA expression was observed after in situ hybridization.</p><p><b>RESULTS</b>During molar development Msx-1 transcripts appeared only in mesenchymal cells, not in epithelial cells. Msx-2 and Dlx-2 both expressed in the epithelial and mesenchymal cells. At the initiation stage of the molar development Msx-2 and Dlx-2 had similar expression. At the bud stage (E13-14) Msx-2 mRNA signaling was intensive in the enamel organ and slight in the dental mesenchyme; Dlx-2 signaling was stronger in the dental papilla. At cap stage (E15-16) Msx-2 showed prominent mRNA signaling in enamel knot and Dlx-2 was maximal in the dental papilla. At the late bell stage (P2-3) Msx-2 transcripts were observed in odontoblasts but not labeled in ameloblasts, and Dlx-2 transcripts appeared in ameloblasts but no labeling was seen in odontoblasts.</p><p><b>CONCLUSIONS</b>Msx-1, Msx-2 and Dlx-2 are expressed in various patterns during murine mandibular first molar development, suggesting they possibly play a role in the interaction between the epithelium and mesenchyme during the molar development.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Transcripción
/
ARN Mensajero
/
Embriología
/
Genes Homeobox
/
Proteínas de Homeodominio
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas de Unión al ADN
/
Factor de Transcripción MSX1
/
Genética
/
Mandíbula
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2003
Tipo del documento:
Artículo
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