Presence of donor-and-recipient-derived DNA microchimerism in the cell-free blood samples of renal transplantation recipients associates with the acceptance of transplanted kidneys / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 477-482, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-253807
ABSTRACT
<p><b>AIM</b>To examine whether the existence of the donor-and recipient-derived DNA chimerism in recipient's plasma can be a predictive marker for the status of transplanted organ.</p><p><b>METHODS</b>One hundred and twenty-six female patients who had been transplanted with male kidneys were enrolled in the present study. In these female recipients, the SRY(1), DYZ(1)(1st) and DYZ(1)(2nd) genes on the Y chromosome from the plasma were prospectively examined using reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>SRY, DYZ(1)(1st) and DYZ(1)(2nd) sequences were detected in the cell-free blood (plasma) of 97 (77%) of 126 female patients with male kidney. The average time that the transplanted kidneys functioned was 8.7 years and 5.4 years among microchimerism-positive and microchimerism-negative recipients, respectively. The frequency of the patients who had acute rejection after renal transplantation was approximately 10% and 28% in microchimerism-positive and microchimerism-negative recipients, respectively. Serum creatinine levels in microchimerism-positive patients were significantly lower than those in microchimerism-negative patients.</p><p><b>CONCLUSION</b>These results suggest that plasma DNA microchimerism present in certain patients following renal transplantation and measurement of plasma DNA microchimerism using quantitative RT-PCR might be a useful predictor for the acceptance of transplanted kidneys.</p>
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Donantes de Tejidos
/
Sangre
/
ADN
/
Secuencia de Bases
/
Sistema Libre de Células
/
Quimera
/
Factores Sexuales
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Trasplante de Riñón
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Asian Journal of Andrology
Año:
2006
Tipo del documento:
Artículo
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