Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 287-289, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-254079
ABSTRACT
<p><b>OBJECTIVE</b>To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.</p><p><b>METHODS</b>TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.</p><p><b>RESULTS</b>TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.</p><p><b>CONCLUSION</b>HBX protein could be induced into mouse liver by TAT induced peptide.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Proteínas Recombinantes de Fusión
/
Expresión Génica
/
Transactivadores
/
Membrana Celular
/
Transporte de Proteínas
/
Proteínas Fluorescentes Verdes
/
Escherichia coli
/
Productos del Gen tat del Virus de la Inmunodeficiencia Humana
/
Proteínas Reguladoras y Accesorias Virales
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS