Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 247-250, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-254092
ABSTRACT
<p><b>OBJECTIVE</b>To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain.</p><p><b>METHODS</b>Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.</p><p><b>RESULTS</b>The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.</p><p><b>CONCLUSION</b>Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Rabia
/
Virus de la Rabia
/
Proteínas Virales
/
Virología
/
Clonación Molecular
/
Vectores Genéticos
/
Genética
/
Metabolismo
/
Métodos
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Artículo
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