The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 (H5N1) / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 180-182, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-254111
ABSTRACT
<p><b>OBJECTIVE</b>To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1.</p><p><b>METHODS</b>The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology. The candidate genes were selected and confirmed by quantitative real-time PCR.</p><p><b>RESULTS</b>The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well.</p><p><b>CONCLUSIONS</b>Our results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Virología
/
Apoptosis
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Perfilación de la Expresión Génica
/
Línea Celular Tumoral
/
Gripe Humana
/
Subtipo H1N1 del Virus de la Influenza A
/
Subtipo H5N1 del Virus de la Influenza A
/
Genética
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Artículo
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