Cloning of PD-1 gene and its prokaryotic expression in Escherichia coli / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 33-35, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-254149
ABSTRACT
<p><b>OBJECTIVE</b>To clone human PD-1 gene, construct a prokaryotic expression plasmid and express in E. coli.</p><p><b>METHODS</b>The human PD-1 cDNA was cloned by RT-PCR from the total RNA, which was extracted from peripheral blood lymphocyte cell of the patient with chronic hepatitis B. Recombinant PD-1 protein was been expressed and purified after the prokaryotic expression plasmid had been constructed. It was identified by SDS-PAGE, DNA sequencing and amino acid sequencing.</p><p><b>RESULTS</b>The PD-1 gene was cloned and confirmed by DNA sequencing. The recombinant protein was expressed in E. coli. The purified protein was obtained, then been confirmed by amino acid sequencing.</p><p><b>CONCLUSION</b>The human PD-1 gene was successfully cloned and expressed in E. coli, which lays the foundation for further study on the function and application of PD-1.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Procariotas
/
Antígenos CD
/
Química
/
Reacción en Cadena de la Polimerasa
/
Alineación de Secuencia
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Análisis de Secuencia de ADN
/
Electroforesis en Gel de Poliacrilamida
/
Escherichia coli
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Artículo
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