Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 933-938, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-254165
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of endogenous leukemia inhibitory factor (LIF) in neonatal rats with periventricular leukomalacia (PVL).</p><p><b>METHODS</b>A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia (HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP.</p><p><b>RESULTS</b>At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group (P<0.01). In the PVL group, the LIF protein level on the third day after HI reached a peak and was higher than the other time points (P<0.01). The change of LIF mRNA expression showed the same tendency with LIF protein. The double staining immunofluorescence showed a co-expression of LIF and GFAP.</p><p><b>CONCLUSIONS</b>LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Leucomalacia Periventricular
/
ARN Mensajero
/
Ratas Wistar
/
Modelos Animales de Enfermedad
/
Factor Inhibidor de Leucemia
/
Genética
/
Proteína Ácida Fibrilar de la Glía
/
Animales Recién Nacidos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2014
Tipo del documento:
Artículo
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