Multidrug resistant effect of alternative splicing form of MAD2 gene-MAD2beta on human gastric cancer cell / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 201-204, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-254342
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of alternative splicing form -MAD2beta of mitotic arrest deficient protein 2 (MAD2) on the formation of multidrug resistance in human gastric adenocarcinoma cell SGC7901.</p><p><b>METHODS</b>RNA was extracted from a multidrug resistance cell line SGC7901/ADR. The full-length MAD2beta cDNA was obtained by RT-PCR and cloned into the pUCm-T vector, and then recombined into the eukaryotic expression vector pcDNA3.1 in forward direction. Subsequently, pcDNA3.1/MAD2beta vectors were then transfected into SGC7901 cells by lipofectamine. Sensitivity to drug was detected by MTT assay. Cell cycle alteration and intracellular fluorescence intensity were determined by FACS.</p><p><b>RESULTS</b>A fragment of 0.53 Kb was obtained and confirmed by DNA sequencing which was a new alternative splicing form of MAD2 named as MAD2beta. pcDNA3.1/MAD2beta transfected SGC7901 cells (SGC7901/MAD2beta) were more resistant to ADR, VCR and MMC than the control cells (SGC7901/pcDNA3.1), and also ADR fluorescence intensity of SGC7901/MAD2beta cells was lower (P < 0.05) than that of SGC7901/pcDNA3.1 cells.</p><p><b>CONCLUSION</b>MAD2beta could increase the multidrug resistance of SGC7901 cell line.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Proteínas Represoras
/
Neoplasias Gástricas
/
Vincristina
/
Proteínas de Unión al Calcio
/
Transfección
/
Adenocarcinoma
/
Doxorrubicina
/
Transactivadores
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2004
Tipo del documento:
Artículo
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