Sphingosine kinase regulates hepatocyte growth factor-induced migration of endothelial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 230-234, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-254557
ABSTRACT
<p><b>AIM</b>To elucidate the effect of sphingosine kinase (SPK) on the hepatocyte growth factor (HGF)-induced migration of endothelial cells.</p><p><b>METHODS</b>We constructed recombinant adenoviral vectors, which contain SPK gene and its mutant respectively. These adenoviral vectors were packaged and amplified in 293 cells. And intracellular SPK activity was assayed via measurement of [32]P radioisotope labeled S1P; the effect of SPK activation on HGF-induced migration of endothelial cell was observed by Transwell technique.</p><p><b>RESULTS</b>Adenoviral mediated expression of SPK gene increased in ECV 304 cells intracellular SPK activity, which in turn enhanced the HGF-induced migration. Whereas these activities were blocked by the dominant negative SPK gene.</p><p><b>CONCLUSION</b>These findings show that SPK activation plays important roles in the regulation of HGF-induced migration of endothelial cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Transducción de Señal
/
Línea Celular
/
Movimiento Celular
/
Adenoviridae
/
Factor de Crecimiento de Hepatocito
/
Fosfotransferasas (Aceptor de Grupo Alcohol)
/
Biología Celular
/
Células Endoteliales
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2006
Tipo del documento:
Artículo
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