Role of TNF-alpha in vascular endothelial cells injury mediated by frozen/thawed PMN / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 393-396, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-254650
ABSTRACT
<p><b>AIM</b>To investigate the role of TNF-alpha in vascular endothelial cells injury mediated by freezing/thaw ing PMN.</p><p><b>METHODS</b>Freezing/thawing cell model was founded using rat PMN isolated by dextran sedimentation technique and VEC cultured in vitro. The injury level of VEC was indicated by measuring activity of LDH in medium. The number of frozen/thawed PMN adhering to VEC was counted with Phagocytizing reactive dyes the degree of frozen/thawed PMN and VEC adhesion. Expression of LFA-1 on the surface of frozen/thawed PMN was analyzed with flow cytometry.</p><p><b>RESULTS</b>TNF-alpha could obviously upregulate expression of LFA-1 on surfaced of frozen/thawed PMN. Upregulation of LFA-1 expression promoted adhesion of frozen/thawed PMN and normal VEC,and aggravated VEC injury. Monoclonal antibody against LFA-1 could partly block adhesion of frozen/thawed PMN and normal VEC,and attenuate VEC injury.</p><p><b>CONCLUSION</b>TNF-alpha can promote expression of LFA-1 on surface of frozen/thawed PMN adhering of frozen/thawed PMN to normal VEC and VEC injury increase, monoclonal antibody against LFA-1 could partly block PMN-VEC adhesion and attenuate VEC injury.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Endotelio Vascular
/
Adhesión Celular
/
Células Cultivadas
/
Antígeno-1 Asociado a Función de Linfocito
/
Factor de Necrosis Tumoral alfa
/
Biología Celular
/
Células Endoteliales
/
Congelación
/
Metabolismo
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2005
Tipo del documento:
Artículo
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