Experimental study on induction of the normal human melanocytes apoptosis in vitro by cycloheximide and TNF-alpha / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 302-305, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-255047
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of cycloheximide and TNF-alpha on melanocyte (MC).</p><p><b>METHODS</b>Melanocyte apoptosis was studied with MTT, transmitting electron microscopy and fluorescence labeling of alive cells.</p><p><b>RESULTS</b>We added TNF-alpha and cycloheximide in melanocytes, and the typical apoptosis appeared 24 hours later, with chromatin condensation, nuclear pyknosis and apoptotic bodies formation. The results of cytometry showed the typical apoptotic peak.</p><p><b>CONCLUSION</b>TNF-alpha and cycloheximide together could inhibit MC proliferation and induce MC apoptosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Técnicas In Vitro
/
Factor de Necrosis Tumoral alfa
/
Apoptosis
/
Cicloheximida
/
Biología Celular
/
Proliferación Celular
/
Sinergismo Farmacológico
/
Melanocitos
Límite:
Adolescente
/
Adulto
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Plastic Surgery
Año:
2005
Tipo del documento:
Artículo
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