Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer / 南方医科大学学报
Journal of Southern Medical University
;
(12): 570-572, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-255251
ABSTRACT
<p><b>OBJECTIVE</b>To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.</p><p><b>METHODS</b>A plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.</p><p><b>RESULTS</b>The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells.</p><p><b>CONCLUSION</b>HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias de la Mama
/
ARN Mensajero
/
Transfección
/
Western Blotting
/
Apoptosis
/
Receptor ErbB-2
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
ARN Interferente Pequeño
/
Interferencia de ARN
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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