Effect of estrogen on osteoprotegerin, osteoclast differentiation factor and macrophage colony stimulating factor mRNA expressions in ovariectomized rat bone tissue / 南方医科大学学报
Journal of Southern Medical University
;
(12): 532-534, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-255261
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of the estrogen on the mRNA expression of osteoprotegerin (OPG), osteoclast differentiation factor (ODF) and macrophage colony stimulating factor (M-CSF) in bone tissue of ovariectomized rats, and investigate the possible pathway of estrogen in preventing and treating postmenopausal osteoporosis. METHODS; Thirty healthy adult SD rats were randomly divided into sham operation group, ovariectomized group and estrogen-treated group. All rats were ovariectomized except those in the sham operation group. Bone density of the L3-L6 vertebra was detected 12 weeks after the operation. The total RNA were extracted from the femur to examine mRNA expression of OPG, ODF and M-CSF by real-time PCR.</p><p><b>RESULTS</b>Estrogen increased the bone density of the ovariectomized rat lumbar vertebra and up-regulated the expression of OPG, whereas down-regulated the expression of M-CSF and lowered ODFOPG ratio.</p><p><b>CONCLUSION</b>The effect of estrogen in treating postmenopausal osteoporosis is closely correlated with the regulation of OPG and M-CSF expressions and ODFOPG ratio.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoporosis
/
Farmacología
/
Huesos
/
ARN Mensajero
/
Ovariectomía
/
Factor Estimulante de Colonias de Macrófagos
/
Ratas Sprague-Dawley
/
Usos Terapéuticos
/
Quimioterapia
/
Estrógenos
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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