Construction and identification of genomic cDNA subclones of dengue 2 virus NGC strain / 南方医科大学学报
Journal of Southern Medical University
;
(12): 469-471, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-255282
ABSTRACT
<p><b>OBJECTIVE</b>To construct the cDNA subclones spanning the entire genome of dengue 2 virus NGC strain for further construction of full-length infectious viral cDNA clone.</p><p><b>METHODS</b>Two pairs of primers were designed according to the restriction endonuclease sites in the viral genome of dengue 2 virus NGC strain. After viral RNA extraction from the brain of infected new-born mice, two parts of full-length viral cDNA were amplified by long RT-PCR and cloned into the vector pCR-XL-TOPO. The partial sequence of the recombinant plasmid was determined.</p><p><b>RESULTS AND CONCLUSION</b>Sequence analysis and digestion with restriction enzymes demonstrated that the two cDNA subclones were specific for dengue 2 virus NGC strain, suggesting the successful construction of the two cDNA subclones of dengue 2 virus NGC strain.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Recombinación Genética
/
Virología
/
Encéfalo
/
ADN Viral
/
ARN Viral
/
Clasificación
/
Genoma Viral
/
Clonación Molecular
/
ADN Complementario
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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