Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies / 南方医科大学学报
Journal of Southern Medical University
;
(12): 290-293, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-255330
ABSTRACT
<p><b>OBJECTIVE</b>To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins.</p><p><b>METHODS</b>BALB/c mice were immunized with the recombinant N proteins of SARS-CoV, 229E and OC43 to obtain the mAbs by means of hybridoma. Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay. Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs.</p><p><b>RESULTS</b>The mAbs against the recombinant N proteins of SARS-CoV, 229E and OC43 were obtained, which reacted specifically with the corresponding viral N protein as shown by indirect ELISA, Western blotting and indirect immunofluorescence assay. No cross-reactivity was found between the 3 N proteins.</p><p><b>CONCLUSION</b>The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes
/
Ensayo de Inmunoadsorción Enzimática
/
Western Blotting
/
Técnica del Anticuerpo Fluorescente Indirecta
/
Proteínas de la Nucleocápside
/
Reacciones Cruzadas
/
Coronavirus Humano 229E
/
Coronavirus Humano OC43
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Alergia e Inmunología
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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