The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells / 대한악안면성형재건외과학회지
Maxillofacial Plastic and Reconstructive Surgery
;
: 7-2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-25547
ABSTRACT
BACKGROUND:
This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor.METHODS:
Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group.RESULTS:
Alkaline phosphatase (ALP) activity and ALP messenger RNA (mRNA) expression level were higher in the strain group than those in the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group compared to those in the control group on days 7 and 14. The vascular endothelial growth factor (VEGF) mRNA expression was higher in the strain group in comparison to that in the control group. Concentration of alizarin red S corresponding to calcium content was higher in the strain group than in the control group.CONCLUSIONS:
The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal-derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Células Madre
/
ARN Mensajero
/
Osteocalcina
/
Osteonectina
/
Calcio
/
Reactores Biológicos
/
Ingeniería de Tejidos
/
Factor A de Crecimiento Endotelial Vascular
/
Fosfatasa Alcalina
Límite:
Humanos
Idioma:
Inglés
Revista:
Maxillofacial Plastic and Reconstructive Surgery
Año:
2017
Tipo del documento:
Artículo
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