Ultrastructural analysis of glioma stem cells-progenitors / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 663-667, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-255607
ABSTRACT
<p><b>OBJECTIVE</b>It is well known that glioma stem cells-progenitors (GSCP) proliferate indefinitely and hardly differentiate in vitro, however, the reasons remain unknown. The aim of this study was to explore the ultrastructural basis of GSCP.</p><p><b>METHODS</b>GSCP, kept by our laboratory, were collected, embedded, and cut into ultrathin sections and observed under the transmission electron microscope.</p><p><b>RESULTS</b>A single GSCP usually had relatively well developed mitochondria, Golgi apparatuses, ribosomes, and undeveloped rough endoplasmic reticulum, but seldom lysosomes and no typical autophagosomes were found, and the nuclear-cytoplasmic ratio was high. The nuclei frequently contained huge amounts of euchromatin and a small quantity of heterochromatin, and in most nuclei there were only one nucleolus, however, two or more nucleoli were also common. Typical apoptotic cells could hardly be found in tumor-spheres, and between neighboring cells in tumor-spheres there were incompletely developed desmosomes or intermediate junction.</p><p><b>CONCLUSION</b>The ultrastructural features of glioma stem cells-progenitors showed that BTSCP were very primitive and the lack of autophagy and the underdevelopment of some other cellular organelles are probably the reasons for the differential inhibition of GSCPs.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Madre Neoplásicas
/
Neoplasias Encefálicas
/
Cromatina
/
Membrana Celular
/
Núcleo Celular
/
Citoplasma
/
Línea Celular Tumoral
/
Microscopía Electrónica de Transmisión
/
Glioma
/
Uniones Intercelulares
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2008
Tipo del documento:
Artículo
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