Preparation and evaluation of a time-resolved fluoroimmunoassay kit for CA50 determination / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 189-192, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-255688
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a rapid and sensitive diagnostic kit for detection of CA50 based on time-resolved fluoroimmunoassay.</p><p><b>METHODS</b>A sandwich-TRFIA diagmostic kit was developed using anti-CA50 monoclonal antibody and all parameters of the kit were evaluated.</p><p><b>RESULTS</b>The linear measurement range of the kit was (5 - 300) U/ml. The sensitivity was 0.2 U/ml. The intra- and inter-assay coefficients of variation were 4.3% - 8.2% and 7.7% - 11.2%, respectively. There was no cross-reaction with CEA, CA12-5, CA15-3 and AFP. The cross reactivity with CA19-9 was 0.7 U/ml. The correlation coefficient of detection results of 107 blood samples between this newly developed kit and commercially available CA50 RIA kit was 0.901.</p><p><b>CONCLUSION</b>This newly developed CA50-TRFIA kit is a valuable test tool for clinical application with even better sensitivity, specificity and accuracy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Estándares de Referencia
/
Sangre
/
Radioinmunoensayo
/
Antígenos de Carbohidratos Asociados a Tumores
/
Fluoroinmunoensayo
/
Reproducibilidad de los Resultados
/
Métodos
Límite:
Adolescente
/
Adulto
/
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2007
Tipo del documento:
Artículo
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