Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2256-2259, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-255802
ABSTRACT
<p><b>BACKGROUND</b>Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. dl-praeruptorin A (Pd-Ia), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-Ia effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-Ia on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action.</p><p><b>METHODS</b>Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry.</p><p><b>RESULTS</b>Pretreatment with i.v. infusion of Pd-Ia prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-Ia at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61 478 +/- 10 074 to 177 408 +/- 10 395 and 195 784 +/- 20 057, and vimentin from 59 189 +/- 19 853 to 164 781 +/- 19 543 and 185 696 +/- 20 957 (P < 0.01, vs placebo), respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin.</p><p><b>CONCLUSION</b>Pd-Ia protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia.</p>
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Vimentina
/
Medicamentos Herbarios Chinos
/
Inmunohistoquímica
/
Daño por Reperfusión Miocárdica
/
Química
/
Western Blotting
/
Calcio
/
Ratas Wistar
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2007
Tipo del documento:
Artículo
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