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The role of Toll-like receptor 4 on inflammation and Aβ formation in cortex astrocytes / 生理学报
Acta Physiologica Sinica ; (6): 631-638, 2014.
Artículo en Inglés | WPRIM | ID: wpr-255992
ABSTRACT
To investigate the role and possible molecular mechanism of astrocytes in inflammation and amyloid β-protein (Aβ) formation, in this research, by using LPS to stimulate cultured rat astrocytes in vitro with or without anti-Toll-like receptor 4 (TLR4) antibody pretreatment, we first detected the TLR4, TNF-α, IL-1β, β-amyloid precursor protein (β-APP) and β-site APP clearing enzyme 1 (BACE1) mRNA with real-time PCR, and TLR4, NF-κB/P65 protein in cultured astrocytes by Western blot, and then further probed the translocation of NF-κB/P65 using immunofluorescence and the contents of TNF-α, IL-1β and Aβ in culture supernatant through ELISA. We found that all of these indexes increased at different degrees after LPS-stimulation. However, if pretreatment with anti- TLR4 antibody, such stimulating effects of LPS on the nuclear translocation of NF-κB/P65 and TNF-α, IL-1β, Aβ contents in astrocytic culture supernatant were reduced significantly or disappeared in comparison with the group with only LPS-administration. Our results suggest that TLR4 in astrocytes might play an important role in the inflammation and Aβ formation through the TLR4/NF-κB signaling pathway, thus providing new knowledge and understanding of the inflammatory hypothesis of AD pathogenesis.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ARN Mensajero / Transducción de Señal / Células Cultivadas / Corteza Cerebral / Astrocitos / Ácido Aspártico Endopeptidasas / Factor de Necrosis Tumoral alfa / Precursor de Proteína beta-Amiloide / Biología Celular / Factor de Transcripción ReIA Límite: Animales Idioma: Inglés Revista: Acta Physiologica Sinica Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ARN Mensajero / Transducción de Señal / Células Cultivadas / Corteza Cerebral / Astrocitos / Ácido Aspártico Endopeptidasas / Factor de Necrosis Tumoral alfa / Precursor de Proteína beta-Amiloide / Biología Celular / Factor de Transcripción ReIA Límite: Animales Idioma: Inglés Revista: Acta Physiologica Sinica Año: 2014 Tipo del documento: Artículo