Amplification, cloning and sequence analysis of spider dragline silk cDNA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 641-643, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-256146
ABSTRACT
Spider dragline silk is synthesized in special gland named major ampulate (MA) gland. The MA glands were dissected from the abdomen of the spiders Nephila clavata and the total RNA was extracted by the TRIZOL. The cDNA of dragline silk was amplificated by RT-PCR (reverse transcription polymerase chain reaction), multiplex PCR and cloned. PCR identification, restriction analysis and DNA sequence analysis were carried out to verify the recombinant plasmids. The codon usage frequencies of the cloned cDNA were added up, and the predicted amino acid sequence was compared with Spidroin2 of Nephila clavipes. Predicted secondary structure of the predicted amino-acid sequence was analysized by DNAStar software. All results showed that the cloned cDNA we got (GenBank Accession No. AF441245) was the very fragment of spider dragline silk Spidroin2 cDNA.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Arañas
/
Codón
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Proteínas
/
Química
/
Reacción en Cadena de la Polimerasa
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Estructura Secundaria de Proteína
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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