Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 626-629, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-256150
ABSTRACT
The effect of the calcium ion (Ca2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2 mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8%, 30.8%, 5.1%, and 26.8%, respectively. However, the Ca2+ concentrations in the medium above 0.6 mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Queratinocitos
/
Adhesión Celular
/
Diferenciación Celular
/
División Celular
/
Células Cultivadas
/
Calcio
/
Senescencia Celular
/
Biología Celular
/
Epidermis
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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