Cloning of the major antigen region of E2 gene of hog cholera virus and expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 605-608, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-256155
ABSTRACT
The major antigen region of E2 gene of Hog Cholera Prevalent Strain (Guangxi Yuling Strain) and Chinese Hog Cholera Lapinised Virus (C-strain) derived from hog and rabbit spleen tissue, was amplified by reverse transcription polymerase chain reaction(RT-PCR) and the nested Polymerase Chain Reaction (nPCR). After the amplified fragments were cloned into the expression vector pPROEX-HTb, the recombinant plasmids pPROEX-GXYL and pPROEX-C were obtained. The insert position, the size and the reading frame were right by PCR, restriction digestion and the sequence analysis. SDS-PAGE indicated that both of the reciepient germs transducted and induced by the recombinant plasmids pPROEX-GXYL and pPROEX-C could express the major antigen region of E2 gene. Western-blot indicated that the expressed antigen protein could be recognized by the positive serum of CSFV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes
/
Proteínas del Envoltorio Viral
/
Western Blotting
/
Reacción en Cadena de la Polimerasa
/
Clonación Molecular
/
Alergia e Inmunología
/
Escherichia coli
/
Genética
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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