Expression of cre gene in Escherichia coli and bioassay its expression product / 生物工程学报
Chinese Journal of Biotechnology
; (12): 497-500, 2002.
Article
en Zh
| WPRIM
| ID: wpr-256177
Biblioteca responsable:
WPRO
ABSTRACT
The Cre recombinase from bacteriophage P1 can recognize specific DNA sequences, cleave DNA at specific target sites, and then ligate it to the cleaved DNA of a second site. In this study, cre gene was cloned into the pGEM-T Easy vector via PCR procedure. Then the cre gene was inserted into an expression vector pET-29a and expressed in E. coli BL21 (DE3). A 38 kD soluble protein was expressed and named CRE. CRE was purified by DEAE-52 chromatography. Bioassay of the partially purified product showed that CRE can cleave the plasmid pGLGFP which contains two loxP site with the same direction.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Plásmidos
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Proteínas Virales
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Proteínas Recombinantes
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Regulación Enzimológica de la Expresión Génica
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Cromatografía DEAE-Celulosa
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Integrasas
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Proteínas Fluorescentes Verdes
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Escherichia coli
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Genética
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Proteínas Luminiscentes
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Article