Expression of polyprotein of infectious bursal disease virus in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 472-476, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-256182
ABSTRACT
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bombyx
/
Proteínas Virales
/
Virología
/
Ensayo de Inmunoadsorción Enzimática
/
Expresión Génica
/
Western Blotting
/
Virus de la Enfermedad Infecciosa de la Bolsa
/
Poliproteínas
/
Electroforesis en Gel de Poliacrilamida
/
Vectores Genéticos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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