Detection of P53 and K-ras gene mutations in lung cancer with oligonucleotide chip / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 447-451, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-256187
ABSTRACT
Different factors including hybridization solution components, hybridization temperature, and the concentration and proportion of the labelled primer, which affected the sensitivity and specificity of single mutation identification, were exploited. Asymmetric PCR increased the hybridization sensitivity, and the asymmetric multi-PCR did not affect the specificity, while the sensitivity was improved a little. Among 30 lung cancer samples detected with the oligonucleotide microarray, 12 was found P53 gene mutations and 5 had K-ras gene mutations. The P53 gene mutations identified by the oligonucleotide microarray was proved 80% same as the sequencing results. The obvious statistical relations of K-ras and P53 gene mutations with tumor type, tumor stage and smoking were not obtained because of less samples and mutation sites.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligonucleótidos
/
Patología
/
Proteína p53 Supresora de Tumor
/
Genes ras
/
Sensibilidad y Especificidad
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Genética
/
Neoplasias Pulmonares
/
Métodos
/
Mutación
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2002
Tipo del documento:
Artículo
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