Determination of flavoniod-glycosides in Yixintong pills by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills.</p><p><b>METHOD</b>Agilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30341203) as mobile phase. Detection wavelength was 330 nm.</p><p><b>RESULT</b>Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively.</p><p><b>CONCLUSION</b>This method can be used for quality control of Yixintong pills.</p>