Effect of puerarin on L-type calcium channel in isolated rat ventricular myocytes / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of Puerarin on L-type calcium channel in isolated rat ventricular myocytes.</p><p><b>METHOD</b>The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion techniques at constant flow rate. Whole-cell recording of patch-clamp techniques was used to observe the current of L-type calcium channel.</p><p><b>RESULT</b>Puerarin 2.4 mmol x L(-1) could inhibit the current of L-type calcium channel of rat ventricular myocytes and this inhibition was time-dependent. Purerarin elevated the current-voltage (I-V) curve of calcium current.</p><p><b>CONCLUSION</b>Puerarin can inhibit L-type calcium current of rat ventricular myocytes. Which implies that puerarin takes part in anti-myocardial ischemia and anti-arrhythmics partly due to the inhibition of L-type calcium channel.</p>