Extraction of coumarins from Angelica dahurica by supercritical extraction and GC-MS analysis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To extract coumarins from Angelica Dahurica by supercritical CO2.</p><p><b>METHOD</b>Six-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. Constituents of the coumarins were analyzed by GC-MS, and their relative contents were determined by normalization method of areas.</p><p><b>RESULT</b>Total 15 coumarins were separated, the relative contents of oxyimperatorin, imperatorin and isoimperatorin being up to 42.40%, 22.14% and 12.12% respectively. Optimum extraction process was established 21 MPa as extraction pressure, 50 degrees C as extraction temperature, 3 h as time for extraction, 20 mesh as pulverized degree, 6.5 MPa as separation pressure and 30 degrees C as separation temperature.</p><p><b>CONCLUSION</b>Changes in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably.</p>