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Effect of methamphetamine exposure on S-nitrosylation of protein disulphide isomerase in PC12 cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 93-96, 2017.
Artículo en Chino | WPRIM | ID: wpr-256511
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of methamphetamine (METH) exposure on S-nitrosylation of protein disulphide isomerase and the neurotoxicity of METH in PC12 cells.</p><p><b>METHODS</b>PC12 cells were exposed to different concentrations of METH, and the cell viability was assessed using the cell-counting kit-8. PC12 cells exposed to METH in the presence of the NOS inhibitor N-nitro-L-arginine (L-NNA) were examined for cell viability and S-nitrosylation of protein disulphide isomerase using the biotin-switch method, and the changes in cell morphology were examined with HE staining.</p><p><b>RESULTS</b>METH exposure obviously decreased the cell viability and increased S-nitrosylation of protein disulphide isomerase, and the effect of METH was obviously inhibited by L-NNA treatment.</p><p><b>CONCLUSION</b>METH can cause obvious neurotoxicity and promote S-nitrosylation of protein disulphide isomerase in PC12 cells.</p>
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Journal of Southern Medical University Año: 2017 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Journal of Southern Medical University Año: 2017 Tipo del documento: Artículo