Quantitative Analysis of DNA Single-strand Breaks in EL 4 cells and Mouse Spleen Lymphocytes after Irradiation / 대한치료방사선과학회지
Journal of the Korean Society for Therapeutic Radiology
;
: 137-144, 1990.
Artículo
en Inglés
| WPRIM
| ID: wpr-25661
ABSTRACT
The filter elution technique was used to assay Co-60 g ray-induced DNA single-strand breaks (SSB) in EL 4 mouse leukemia cell and spleen lymphocyte. The lymphocytes were stimulated with lipopolysaccharide (LPS, 20 mug/ml) to label [3H] thymidine. EL 4 cells and lymphocytes in suspension were exposed at 0degree C to gy, 1 gy, 5 gy, 10 gy of Co-60 radiation and elution procedure was performed at PH 12.1. The number of DNA single-strand breaks increased with increasing doses of g rays. The strand scission factor (SSF) was estimated in each experiment (eluted volume 21 ml. The slope for EL 4 cells was 0.01301+/-0.00096 gy-1(n=5) and the slope for lymphocytes was 0.01097+/-0.00091 gy-1(n=5). The slopes were significantly different (p<0.005). Thus EL 4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Radiación Ionizante
/
Bazo
/
Timidina
/
ADN
/
Linfocitos
/
Leucemia
/
Concentración de Iones de Hidrógeno
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of the Korean Society for Therapeutic Radiology
Año:
1990
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS