Identification of medicinal plant Dendrobium based on the chloroplast psbK-psbI intergenic spacer / 药学学报
Acta Pharmaceutica Sinica
;
(12): 783-787, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-257067
ABSTRACT
In this paper, the chloroplast psbK-psbI intergenic spacers of 18 species of Dendrobium and their adulterants were amplified and sequenced, and then the sequence characteristics were analyzed. The sequence lengths of chloroplast psbK-psbI regions of Dendrobium ranged from 474 to 513 bp and the GC contents were 25.4%-27.6%. The variable sites were 71 while the informative sites were 46. The inter-specific genetic distances calculated by Kimura 2-parameter (K2P) of Dendrobium were 0.006 1-0.058 1, with an average of 0.028 4. The K2P genetic distances between Dendrobium species and Bulbophyllum odoratissimum were 0.093 2-0.120 4. The NJ tree showed that the Dendrobium species can be easily differentiated from each other and 6 samples of the inspected Dendrobium species were identified successfully through sequencing the psbK-psbI intergenic spacer. Therefore, the chloroplast psbK-psbI intergenic spacer can be used as a candidate marker to identify Dendrobium species and its adulterants.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Cloroplastos
/
Clasificación
/
ADN de Plantas
/
ADN de Cloroplastos
/
ADN Espaciador Ribosómico
/
Dendrobium
/
Genética
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2015
Tipo del documento:
Artículo
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