Effects of zinc on bone development in mouse embryo in vitro / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 97-99, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-257298
ABSTRACT
<p><b>OBJECTIVE</b>This study was designed to explore the effects of zinc on bone development.</p><p><b>METHODS</b>Forelimbs of mice with 16 d gestation were cultured by a rotating device.</p><p><b>RESULTS</b>Contents of OC and (45)Ca and activities of AKP in the bone tissues cultured at zinc-deficiency media and at media with 120 micro mol/L of Zn(2+) decreased significantly. Synthesis of OC, absorption of calcium and activities of AKP in the bone tissues cultures at media with 45 and 70 micro mol/L of Zn(2+) increased significantly. Radiograph of bone tissues showed that length of long bone cultured at zinc-deficiency media and at media with 120 micro mol/L of Zn(2+) shortened and their density reduced, and those cultured at media with 45 and 70 micro mol/L of zinc increased, as compared with self-control bone. Histological analysis showed the death of bone cells and loss of stroma in the bone tissues cultured at media with 120 micro mol/L of Zn(2+), and active proliferation and differentiation of bone cells, and secretion and synthesis of osteoid increased in the bone tissues cultured at media with 45 and 70 micro mol/L of zinc.</p><p><b>CONCLUSIONS</b>Adequate supplementation of zinc could promote formation and development of bone tissues and deficiency or excess of zinc could alter their growth and development and normal metabolism.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Zinc
/
Desarrollo Óseo
/
Factor II del Crecimiento Similar a la Insulina
/
Proteínas
/
Densidad Ósea
/
Embriología
/
Calcio
/
Embrión de Mamíferos
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Journal of Preventive Medicine
Año:
2002
Tipo del documento:
Artículo
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