Protective Effect of S-isopentenyl-L-cysteine against DNA Damage in Irradiated Mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 496-500, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-257605
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the protective effect of S-isopentenyl-L-cysteine,a new cysteine derivative,on DNA damage induced by radiation by using acute radiation injury animal models.</p><p><b>METHODS</b>Forty ICR mice were randomly divided into five groupsthe control group,1.0Gy gamma irradiation group,1.0Gy gamma irradiation combined with S-isopentenyl-L-cysteine group,7.2Gy gamma irradiation group,and 7.2Gy gamma irradiation combined with S-isopentenyl-L-cysteine group,with 8 mice in each group.The comet assay and bone marrow polychromatic micronucleus experiments were performed to evaluate the double-strand DNA breaks in ICR mice exposed to 1.0 and 7.2Gy gamma-ray, respectively.</p><p><b>RESULTS</b>The tail DNA percentage,tail length,tail moment,and olive tail moment of peripheral blood lymphocytes in 7.2Gy gamma irradiation group were significantly higher than that of the control group (P<0.01).And it was also observed that above experimental indexes of 7.2Gy gamma irradiation combined with S-isopentenyl-L-cysteine group was significantly less than that of 7.2Gy gamma irradiation group (P<0.05). In addition,the micronucleus rate of 1.0Gy gamma irradiation group and 7.2Gy gamma irradiation group were both significantly higher than in the control group (P<0.01). In addition,in mice given S-isopentenyl-L-cysteine before irradiation,the micronucleus rate of ICR mice exposed to 1.0 and 7.2Gy gamma-ray decreased from (39.5000 ± 3.3141)‰ to (28.1667±4.1345)‰ (P=0.033) and from (76.5000 ± 4.6242)‰ to (22.8333 ± 3.6553)‰(P=0.000),respectively. The bone marrow polychromatic micronucleus experiment indicated that the value of polychromatic erythrocyte (PCE)/normochromatic erythrocyte(NCE) of ICR mice exposed to 1.0 and 7.2Gy gamma-ray was less than the control group(P<0.05). Meanwhile,after irradiating by certain dose,the value of PCE/NCE in mice given S-isopentenyl-L-cysteine before irradiation was significantly higher than the corresponding groups (P<0.05).</p><p><b>CONCLUSION</b>S-isopentenyl-L-cysteine has a good protective effect against DNA damage induced by radiation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Traumatismos por Radiación
/
Protectores contra Radiación
/
Médula Ósea
/
Daño del ADN
/
Cisteína
/
Modelos Animales de Enfermedad
/
Rayos gamma
/
Ratones Endogámicos ICR
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2015
Tipo del documento:
Artículo
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