Nucleic acid quantifying assay of hepatitis B virus / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the relation between the nucleic acid content of hepatitis B virus and hepatitis B virus markers, liver function and its pathological state.</p><p><b>METHODS</b>Thirty-nine patients suffering from chronic hepatitis B with positive HBsAg were performed liver puncture for histopathological examination. Fluorescence ration PCR analysis was used for HBV DNA quantifying assessment. Hepatitis B virus markers were detected by ELISA.</p><p><b>RESULTS</b>Serum HBV DNA content was related to hepatitis B virus markers. There was no correlation between HBV DNA level and the Scheuer grade in patients with positive HBsAg, and no obvious correlation between serum glutamic pyruvic transaminase level and HBV DNA content.</p><p><b>CONCLUSIONS</b>There is a significant correlation between HBeAg and HBV DNA, but not between liver inflammation stage and serum HBV DNA content.</p>