Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 315-322, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-258817
ABSTRACT
<p><b>OBJECTIVE</b>Chlamydia psittaci is an avian respiratory pathogen and zoonotic agent. The wide prevalence of C. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies against C. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen.</p><p><b>METHODS</b>The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens.</p><p><b>RESULTS</b>The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively.</p><p><b>CONCLUSION</b>These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance of C. psittaci infection.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Psitacosis
/
Enfermedades de las Aves de Corral
/
Proteínas Bacterianas
/
Ensayo de Inmunoadsorción Enzimática
/
Pollos
/
Chlamydophila psittaci
/
Sensibilidad y Especificidad
/
Diagnóstico
/
Alergia e Inmunología
/
Genética
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2016
Tipo del documento:
Artículo
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