Enhancement of Virus Replication in An Influenza A Virus NS1-Expresssing 293 Cell Line / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 224-228, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-258830
ABSTRACT
The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virus de la Influenza A
/
Fisiología
/
Cultivo de Virus
/
Replicación Viral
/
Línea Celular
/
Regulación de la Expresión Génica
/
Proteínas no Estructurales Virales
/
Células HEK293
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2016
Tipo del documento:
Artículo
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