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Construction, expression and bioactivity characterization of targeting toxin DT-VEGF / 生物工程学报
Chinese Journal of Biotechnology ; (12): 192-196, 2004.
Artículo en Chino | WPRIM | ID: wpr-259125
ABSTRACT
Tumor rapid growth depends on neovascularization. Vascular endothelial growth factor, the main mediator during the occurrence and formation of vascularization, has specific receptors whose expression rate shows difference of orders of magnitude between tumor and the normal tissue, so it can be used to transport toxin molecules to the proliferative tumor endothelial and kill cancer cells. In our experiment, we constructed fusion protein DT-VEGF by linking diphtheria toxin's forward 389 amino acids's gene and VEGF165 via a linker. DT-VEGF is expressed in E. coli and purified. Our experiment proves in can kill vascular endothelial cells specifically, and the inhibition of neovascularization of chicken chorionic membrane is also confirmed.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Inmunotoxinas / Inhibidores de la Angiogénesis / Factores de Crecimiento Endotelial Vascular / Toxina Diftérica / Escherichia coli / Vectores Genéticos / Genética / Metabolismo Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Inmunotoxinas / Inhibidores de la Angiogénesis / Factores de Crecimiento Endotelial Vascular / Toxina Diftérica / Escherichia coli / Vectores Genéticos / Genética / Metabolismo Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2004 Tipo del documento: Artículo