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Expression of phytase gene in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology ; (12): 112-115, 2003.
Artículo en Chino | WPRIM | ID: wpr-259184
ABSTRACT
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Aspergillus niger / Bombyx / Proteínas Fúngicas / Southern Blotting / 6-Fitasa / Electroforesis en Gel de Poliacrilamida / Vectores Genéticos / Genética / Concentración de Iones de Hidrógeno / Metabolismo Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Aspergillus niger / Bombyx / Proteínas Fúngicas / Southern Blotting / 6-Fitasa / Electroforesis en Gel de Poliacrilamida / Vectores Genéticos / Genética / Concentración de Iones de Hidrógeno / Metabolismo Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2003 Tipo del documento: Artículo