High expression and characterization of human parathyroid hormone in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 102-106, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-259186
ABSTRACT
Human parathyroid hormone (hPTH) was highly expressed in Escherichia coli by inserted the synthesized whole hPTH cDNA into the vectors pBV220 and pET22b. After expression and disruption, the purified product was acquired through cation exchange chromatography and reverse phase chromatography. From the results of N-terminal sequencing and MALDI-TOF-MS analysis the recombiant prtein was indentified as intact hPTH. In in vitro Bioassays the recombinant hPTH stimulated adenylate cyclase as the standard did. In ovariectomized rats the recombinant hPTH markedly increased the femoral bone mass and bone mineral density.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Hormona Paratiroidea
/
Farmacología
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Ovariectomía
/
Densidad Ósea
/
Química
/
Cromatografía por Intercambio Iónico
/
Alineación de Secuencia
/
Secuencia de Aminoácidos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2003
Tipo del documento:
Artículo
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